Glyceryl kinase

The related term substrate specificity cf. Crans, D. C. Whitesides, G. M., /. Am. Chem. Soc., 1985, 107, 7008 Zaks, A. Klibanov, A. M., /. Am. Chem. Soc., 1986, 108, 2767) refers to the productive interaction of a specific substrate with an enzyme, e.g. glyceryl kinase, chymotrypsin, subtilisin. While each substrate is examined individually and the kinetics of each is established separately, it is conceivable that a mixture of two or more substrates, when treated with the same enzyme in a given reaction mixture, would exhibit morpholytic selectivity - the generalized concept discussed in this chapter. 

Commercial kits are based on the Upase-catalyzed total hydrolysis of the triglyceride, followed by glycerol kinase-catalyzed synthesis of 1-glyceryl phosphate (67) and GPO-catalyzed oxidation of the latter, as shown in equation 17. The end analysis is by a chromogenic oxidation process catalyzed by peroxidase, similar to equation 27, involving 4-aminoantipyrine (81) and 3,5-dichloro-2-hydroxybenzenesulfonate (88) or iV-ethyl-iV-(3-sulfopropyl)-m-anisidine (94)28. 290... 

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