Glycerol quenching sodium periodate

Centrifuge and wash cells several times with PBS. Some protocols include a quench step wherein excess sodium periodate is eliminated by addition of glycerol. This can be accomplished by adding 3 volumes of 0.1 M glycerol in PBS prior to centrifugation. 

Quench the reaction by the addition of 0.1 ml of glycerol per milliliter of reaction solution. Alternatively, the reaction may be stopped by immediate gel filtration on a Sephadex G-25 column. The dextran beads of the chromatography support will react with sodium periodate to quench excess reagent. To quench the reaction with cellular samples, wash the cells with buffer to remove remaining traces of periodate. 

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