Glycerol cascade conversion

Fig. 13.6 A m ulti-enzyme one-pot example cascade conversion of glycerol into a heptose sugar through consecutive phosphorylation, oxidation, aldol reaction and dephosphorylation [11],...

Another interesting case is the one-pot four-enzyme cascade conversion of glycerol into a heptose sugar on gram scale [11], in which a pH switch method is applied to temporarily turn off one of the enzymes involved (Fig. 13.6). The four consecutive enzymatic conversion steps in one and the same reactor, without separation of intermediates, consist of ... 

The same as above holds for the (by-product) glycerol, a really challenging molecule for cascade conversions in water. 

MonoSik, R., Ukropcova, D., Stredansky, M., and Sturdik, E. (2012) Multienzy-matic amperometric biosensor based on gold and nanocomposite planar electrodes for glycerol determination in wine. Anal. Biochem., 421, 256-261. Bruggink, A., Schoevaart, R., and Kieboom, T. (2003) Concepts of nature in organic synthesis cascade catalysis and multistep conversions in concert. Org. Process Res. Dev., 7, 622-640. 

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