Glycerides mass spectrometry

Ellerbe PM, Sniegoski LT, and Welch MJ (1995) Isotope dilution mass spectrometry as a candidate definitive method for determining total glycerides and triglycerides in serum. Clin Chem 41 397-404. 

From the shell of A. crassipina has been isolated a sulfoquinovosylmono-glyceride, and its structure has been established by chemical splitting (alkaline methanolysis and acid hydrolysis), as well as by such physicochemical methods as H- and l3C-n.m.r. spectroscopy, g.l.c.-mass spectrometry, and field-desorption mass spectrometry.214 The major component was shown to be l-0-hexadecanoyl-3-0-(6-C-sulfo-a-D-quinovosyl)glycerol (96% of the mixture), and the minor one was its analog containing tetradecanoic acid. 

Ismaiel OA et al (2010) Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 878 3303-3316... 

Figure 9.18 Mass spectrometry of glycerides, phospholipids and fatty adds, (a) origin of main fragmentation ions as illustrated (b) (c) Example of negative ion FAB MS/MS CID mass spectrum of indicated fatty acid. The main fragment ions visible are homologation fragment anions. Mass differences between consecutive fragment anions define fatty acid CH2-length (

DeLuca SJ, Sarver EW, Voorhees KJ. Direct analysis of bacterial glycerides by curie-point pyrolysis-mass spectrometry. J Anal Appl Pyrolysis 1992 23(1) 1-14. 

One of the most important applications of mass spectrometry has always been the determination of molecular mass. Since this can be difficult with EIMS, the search for soft ionization techniques is almost as old as organic mass spectrometry. The first step was chemical ionization and particularly direct chemical ionization (DCl), which allowed the samples to be desorbed directly from a thin wire into the Cl plasma. It has been successfully u,sed for natural compounds such as glycerides. Later, field ionization (FI) and especially field desorption (FD) opened up new possibilities for the analysis of labile natural compounds such as saccharides, fteptides, and nucleotides. When fast... 

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