Glycated proteins in hair

Sample solution is diluted 40 times by 600 mmol/L sodium hydroxide, the diluted sample solution 50 /iL is poured into the plate. After addition of a luminescence solution (40 mmol/L potassium hexacyanoferrate (II ) and 0.1 mmol/L potassium hexacyanoferrate (III) containing 5 /nmol/L luminol solution), luminescence intensity was measured. Calibration curve was obtained by measurement of the chemiluminescence intensity using GPL as a standard, and the relative amount of the glycated protein in hair was computed. 

The glycated protein in hair correlated well with the HbAic in blood (r=0.980)... 

Table 1. Effect of arginine on compensation of glycated protein in hair collected at...

Figure 1, Correlation between glycated protein in hair and HbAic in bl...

The distribution of glycated protein in hair and HbAic in blood over several months is shown in Fig 2. As the results show, it seems to be possible to evaluate past states of diabetes mellitus. The good correlation between these two tests suggests that the proposed method could be useful in monitoring of diabetes mellitus as an alternative to HbAlc in blood. 

DEVELOPMENT OF HIGHLY SENSITIVE ANALYSIS OF GLYCATED PROTEIN IN HUMAN HAIR BY LUMINOL CHEMILUMINESCENCE... 

Highly Sensitive Analysis of Glycated Protein in Human Hair... 

Preparation of sample solution One centimeter hair sample was rinsed with 1 mL of ethanol at 37 °C for 30 min. After removing ethanol using a capillary pipette, the sample was dissolved in 1 mL of 600 mmol/L sodium hydroxide at 95 C for 10 min. Measurement of glycated proteins... 

The correlation of the value of glycated protein by compensation with arginine and the levels of HbAic in blood were examined. In addition, the glycated protein that shifts to the tip of hair due to growth, and past levels of HbAxc were examined. 

Figure 2. Past HbAlc in blood and glycated protein distribution in hair.

---