Glycans incorporating functionalized

Another metal present in biological media in relatively high concentrations is iron. Most iron ions are strongly coordinated within proteins, although a small fraction of iron ions is loosely bound with carboxylates and phosphates, phospholipids, glycans, and sulphonates. This fraction is usually called labile iron and constitutes a pool of iron ions accessible for incorporation into functional metalloproteins. In contrast to closed shell cations, rather than increasing the fluorescence quantum... 

Inhibition of glycan production by MOE. (A) PSA production is terminated upon the sequential incorporation of SiaSBut (shown) or SiaSPent residues. (B) Decoys function as inhibitors of glycosylation by hijacking the metabolic machinery resulting in glycan synthesis on these non-natural initiators rather than on the normal complement of cellular proteins or lipids... 

Figure 4. Literature precedents for the incorporation of functionalized monosaccharides into cellular glycans.

Other multifunctional probes incorporate both a photocross-linker and a purification tag. These reagents have the potential to be used to identify the portion of the lectin that functions in glycan recognition. For example,... 

Fig. 10.3-16 Introduction of unnatural functional groups through posttranslational modification, (a) Ketones and azides can be introduced onto cell surfaces by feeding cells with unnatural sialic acid precursors, such as mannosamine derivatives 40b and c. These are incorporated into cell-surface glycans, which can be further elaborated using additional bioconjugation reactions, (b) Specific amino acid sequences can be modified using biotin ligase. Interestingly, ketobiotin" is also recognized as a substrate for the enzyme, allowing a...

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