Glycans functional analysis

Fig. 1 Glycan array analysis of codakine as measured by fluorescence intensity (glycan array v3.0 at Consortium for Functional Glycomics). Purified codakine from white clams was purified with Alexa Fluor 488 Protein Labeling Kit (Molecular Probes , Invitrogen) and tested on glycan array v3.0 at Consortium for Functional Glycomics.

B. Henrissat A journey through families of carbohydrate-active enzymes Y. Ito Synthesis and functional analysis of glycoprotein glycan chains... 

M. B. Thygesen, J. Sauer, and K. J. Jensen, Chemoseleetive capture of glycans for analysis on gold nanoparticles Carbohydrate oxime tautomers provide functional recognition by proteins, Chem. Eur. J., 15 (2009) 1649-1660. 

In view of the efficient inhibition of a particular crystalline glycan hydrolase by the epoxylbutyl -cellobioside 7 (n = 2), we decided to prepare the deoxy iodo derivative 21 to aid in the X-ray crystallographic analysis. We soon found that, although the alkene 22 was easily available as a direct precursor to our target, the epoxide functionality had to be introduced indirectly using bromo-hydrin 23 technology any direct oxidation of 22 invariably led to some loss of the iodine atom [23]. 

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