Gluten crude

Hydrolysis of Bitter Peptides from Corn Gluten by CPase Top. When a 0.5% bitter peptides solution was incubated with crude CPase Top (enzyme/substrate ratio = 2 /ikat/g), bitterness diminished similarly in a short time, and was almost undetectable after 15 hr (Fig. 3). Liberated amino acids increased similarly as in the other bitter peptide studies. 

Glutamic acid is also prepared by the acid hydrolysis of gluten. In a procedure described by Waters,43 the crude, hydrolyzed product is filtered while hot to remove the insoluble humus, after which the filtrate is cooled to crystallize the glutamic acid hydrochloride. The crystals are dissolved in water, and sufficient sodium hydroxide is added to neutralize the free hydrochloric acid. The solution (pH 1.5) is heated to 80 to 90° C and decolorized with activated carbon. When the filtrate is cool, sufficient sodium hydroxide is added to raise the pH to 3.2 and the solution is then allowed to stand until crystallization is complete. 

Adams and Leslie (3) made a study of the maintenance of optimum pH in mashes fermented with B. polymyxa. Control of this factor is necessary because the organism produces acids which will ultimately stop the fermentation. Acidity may be controlled by addition of calcium carbonate but ammonia would be more desirable as a neutndizer in an industrial process because it is easily handled and does not add to the ash in the residue and thus lessen its feed value. The authors showed the use of ammonia to be practicable. Fratkin and Adams (42) studied the use of wheat from which some of the gluten and other fractions of the grain had been removed to give a crude wheat starch. This material was considered because its use would lessen processing difficulties. The addition of malt sprouts was found to be the most effective fermentation supplement. With these added, the crude starch could be fermented with good yields in about 72 hours. 

The kernel composition is very similar to Yellow Com (in %) - Crude Protein 14 (- gluten-cereal, - zein) fibers 1.9 Mineral Matter 1.9 Starch 68.5 Crude Fat 3.5. 

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