Glutathione peroxidase activation coefficient

Glutathione-peroxidase activity was measured spectrophoto-metrically at 340nm by an enzyme coupled assay procedure of Paglia and Valentine (28) as modified by Reddy, et al. (26). A molar extinction coefficient of 6.2 x 103cm 1 was used in calculations. 

Calculation of enzyme activity The decrease in NADPH concentration, zf(NADPH)/min is calculated from the linear slope of the graph obtained and the appropriate absorption coefficient for NADPH. The true glutathione peroxidase-dependent reaction rate is obtained by subtracting the value of the non-enzymic and hydroperoxide-independent apparent rates. Glutathione peroxidase activity is frequently given simply in terms of d(NADPH)/min under standard conditions. A calculation of an activity value, A, that is likely to be more universally applicable, is given in by Flohe and Gumzler (1984). 

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See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

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