Glutaric acid normal urine

S. Glutaric Acid. Glutaric acid has been found by paper chromatography to be a regular constituent of normal human urine (N20, 05). 

Adipic Acid. Adipic acid was first shown in normal urine during paper chromatographic studies (N13) the daily normal elimination lies between 1.3 and 2.5 mg (T18) but increases after administration of e-aminocaproic acid, a part of which would be transformed into adipic acid by oxidative deamination (T18). After administration of adipic acid, one finds a larger amount in the urine than when administering glutaric acid (W8). 

Methylsuccinic Acid. Methylsuccinic acid has been shown to be present in normal urine in about the same amount as glutaric acid (T15). It increases after administration of leucine. 

Fig. 7.2 Chromatogram of acidic metabolites extracted from the urine of a normal child using DEAE-Sephadex and re-extraction with solvents after ethoxime formation and freeze-drying by reconstitution in water, acidification with hydrochloric acid, saturation with sodium chloride, and solvent extraction with diethyl ether (three times) and ethyl acetate (three times), evaporation of the solvents from the combined extracts using dry nitrogen and trimethylsilylation using the minimum quantity of BSTFA. Separated on 10 per cent OV-101 on HP Chromosorb W (80-100 mesh) by temperature programming from 110°C to 285°C at 4°C min with an initial 5 min isothermal delay. Peak identifications are 1, phenol plus lactate 2, glycollate 3, cresol 4, 3-hydroxyisovalerate 5, benzoate 6, phosphate 7, succinate 8, 3-methyladipate 9, 3-hydroxy-3-methyl-glutarate 10, 4-hydroxyphenylacetate 11, homovanillate plus some aconitate 12, hippurate 13, citrate 14, vanilmandelate 15, n-tetracosane (standard) 16, n-hexacosane (standard).

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