Glutamate-pillared LDHs

A more sophisticated method was developed by Ren et al. [123] for the immobilization of penicillin G acylase by covalent grafting in the interlayer galleries of a LDH by a three-step procedure using a glutamate pillared LDH as the starting material. 

Fig.1 Schematic illustration of the immobilization of penicillin G acylase on glutamate-pillared LDHs. Reaction of the primary amino groups of the intercalated glutamate ions with glutaraldehyde through Schiff s base formation is followed by covalent coupling of the residual aldehyde groups with the free amino groups of the enzyme...

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