Glutamate cerebellum

Obata, K, Fukuda, T, Konishi, S, Ji, FY, Mitoma, H and Kosaka, T (1999) Synaptic localization of the 67,000 mol. wt isoform of glutamate decarboxylase and transmitter function of GABA in the mouse cerebellum lacking the 65,000 mol. wt isoform. Neuroscience 93 1475-1482. 

D.S. Bredt and S.H. Snyder, Nitric-oxide mediates glutamate-linked enhancement of cgmp levels in the cerebellum. Proc. Natl. Acad. Sci. U.S.A. 86, 9030-9033 (1989). 

This observation led to the finding that a soluble enzyme from rat forebrain catalyzes the NADPH-dependent formation of NO and L-citrulline from L-ar-ginine by calcium-dependent mechanisms (Knowles et al., 1989). A subsequent study revealed that glutamate and NMDA stimulate NO and L-citrulline formation in rat cerebellar slices and that this is associated with a concomitant stimulation of cyclic GMP formation (Bredt and Snyder, 1989), thus establishing a link between NMDA-receptor activation and cyclic GMP formation (Fig. 4). These investigators went on to purify and characterize NO synthase from cerebellum (Bredt and Snyder, 1990 Bredt et al., 1991) and to show the localization of NO synthase in the brain to discrete neuronal populations (Bredt et al., 1990). 

Suarez I., Bodega G., and Fernandez B. (2000). Modulation of glutamate transporters (GLAST, GLT-1 and EAAC1) in the rat cerebellum following portacaval anastomosis. Brain Res. 

Favaron M., Manev H., Alho H., Bertolino M., Ferret B., Guidotti A., and Costa E. (1988). Ganglio-sides prevent glutamate and kainate neurotoxicity in primary neuronal cultures of neonatal rat cerebellum and cortex. Proc. Natl. Acad. Sci. USA 85 7351-7355. 

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