Glucuronide chromatograms

Figure 4.4 HPLC chromatogram obtained on a 50 xl injection of human urine collected from 0-4 h after a 1000 mg dose of BW935U83. The locations for BW935U83 (4), its glucuronide conjugate (5), and 3-fluoro-ribolactone (6) are indicated...

Figure 9.70 Chromatogram of the HPLC resolution of a-naphthol glucuronide. Peaks 1, a-naphthol glucuronide 2, /3-naphthol, the internal standard 3, a-naphthol, the substrate. Solvent 0.1 M acetic acid-methanol (55 45, v/v) flow rate 1.5 mL/min wavelength, 240 nm. (From To and Wells, 1984.)...

Figure 5.4 Chromatograms with multiple reaction monitoring (MRM) transitions at 625/287 (A) and 639/301 (B) that show the multiple glucuronide forms of cyaniding-3-glucoside and peonidin-3-glucoside, respectively. (From Hager, 2008).

Figure 3.11 Extracted ion chromatograms for miz 548.12 (glucuronides of midazolam) from the analyses shown in Figure 3.10, for (a) HPLC and (b) UPLC. Note the two metabolites, arising from glucuronidation at different hydroxylation sites in midazolam, that are well-resolved in (b) but not in (a). Reproduced from Plumb et al. Rapid Commun. Mass Spectrom. (2004) 18, 2331, with permission of John Wiley Sons, Ltd.

Figure 10.7 Comparison of a chromatogram obtained for the analyte in a QC sample made from control matrix with that fa- an incurred sample with a co-eluting metabolite (glucuronide). In this case the chromatography conditions were modified and the method was revalidated.

Figure 4 Reversed-phase HPLC elution profiles of tocopherols (panel A), retinoids (B), and carotenoids (C) present in human plasma (200 pL). Blood was collected 3 h after an oral dose of retinoic acid. The chromatogram was obtained by use of gradient elution (Table 3). Peak identification 2, 4-oxo-retinoic acid 4, retinoyl P-glucuronide 7, retinoic acid 8, retinol 9, retinyl acetate (internal standard) 15, butylated hydroxy toluene 16, y-tocopherol 17, a-tocopherol 18, free bilirubin 19, lutein 20, zeaxanthin 21, 2, 3 -anhydrolutein 22, P-cryptoxanthin 23, lycopene 24, a-carotene 25, P-carotene. (From Ref. 73.)...

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