Glucosyltransferase membrane-bound

Ullman, P, Bouvier-Nave, P. and Benveniste, P. (1987) Regulation by phospholipids and kinetic studies of plant membrane-bound UDP-glucose sterol (3-D-glucosyltransferase. Plant Physiol, 85,51-5. 

Wamecke, D. and Heinz, E. (1994) Purification of a membrane-bound UDP-glucoseisterol fJ-D-glucosyltransferase based on its solubility in diethyl ether. Plant Physiol, 105,1067-73. 

The search for the corresponding enzyme vomilenine 21-O-p-D-glucosyltransferase was successful only when microsomal proteins were analyzed for the enzyme activity. The transferase reaction was dependent on active protein in the presence of substrate vomilenine and cosubstrate uridine diphosphate glucose (UDPG) (the orJy one accepted from several tested UDP monosaccharides). However, the transferase was far less specific for the nucleotide part of the cosubstrate. Enzyme activity was only detected in a Rauuolfia cell suspension which produces the glucoalkaloid rau-caffricine (30). Inhibition of this membrane-bound transferase might probably enhance ajmaline production in Rauuolfia which has, however, not yet been proven so far. 

A membrane-bound 2-acetamido-2-deoxy-D-glucosyltransferase isolated from B. megaterium transferred 2-acetamido-2-deoxy-D-glucose from UDP-2-acetamido-2-deoxy-D-glucose to an A -acetylmuramylpentapeptidyl-lipid intermediate (MurA Ac-L-Ala-D-y-Glu-/Me5 o-A2pm-D-Ala-D-Ala-P-P-Iipid). ... 

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