8-Glucosidases kinetic parameters

Rates of hydrolysis of /)-nitrophenyl-P-D-glucopyranoside by P-glucosidase, an irreversible unimolecular reaction, were measured at several concentrations of the substrate. The initial reaction rates were obtained as given in Table 3.2. Determine the kinetic parameters of this enzyme reaction. 

Table 3 Kinetic parameters of native and immobilized P-D-glucosidase at 20°C and 40°C [23] ...

Changed kinetic parameters of the enzyme may be one of the reasons leading to the increased aryl-3-glucosidase in the mutant ATG-5. When comparing K and V in the wild and the mutant strain at 50 C (Table 1) it was shown that So h parameters changed. The enzyme affinity to the substrate increased roughly 2.5-fold in the mutant strain, however, V simultaneously decreased. Thus, it can be assumed that qualitative changes... 

---