Glucose pullulan

Addition of an aqueous solution of PEG to a saturated aqueous solution of a-CD at room temperature did not lead to complex formation unless the average molecular weight of PEG exceeded 200 [46]. Moreover, carbohydrate polymers such as dextran and pullulan failed to precipitate complexes with PEG, and the same was true for amylose, glucose, methyl glucose, maltose, maltotriose, cyclodextrin derivatives, such as glucosyl-a-CD and maltosyl-a-CD, and water-soluble polymers of a-CD crosslinked by epichlorohydrin. These facts suggested to Harada et al. the direction for further research. 

This enzyme [EC 3.2.1.57] catalyzes the hydrolysis of pullulan to produce isopanose (6-a-maltosylglucose). Panose (4-o -isomaltosylglucose) is a substrate and is hydrolyzed to isomaltose and glucose. 

After fractionation with cetylpyridinium chloride, the acidic heteropolysaccharide of Aureobasidium (Pullularia) pullulans contains D-mannose, D-galactose, D-glucose, and D-glucuronic acid in the ratios of 43 35 10 12. It has a main chain of (1— 6)-linked a-D-mannopyrano-syl units substituted as in structure158 38. 

High conversion (70%) of substrate (sucrose) into pullulan makes the polysaccharide attractive economically. Pilot-plant, batch fermentations of D-glucose to yield pullulan have been reported.425 Large-scale, pilot fermentations by Hayashibara Biochemical Laboratories423 are expected to lead to full-scale production of pullulan. 

Cleaves a-1,6 glucose linkages in pullulan, starch glycogen, etc. 

So far, pullulan has been reported to arise from Pullularia spp. only. Ueda and coworkers described formation of viscous solutions from strains of Pullularia pullulans and Pullularia fermentans, and an uncharacterized polysaccharide mixture containing glucose residues was extracted from cells of Pullularia prototropha. ... 

Pullulan-hydrolase Type III pullulan [a-1,4] glucose, maltose maltotriose Thermococcus aggregans... 

Leathers, T. D., Detroy, R. W., Bothast, R. J. (1986). Induction and glucose repression of xylanase from a color variant strain of Aureobasidium pullulans. Biotechnology Letters, 8, 867-872. 

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