Glucose oxidase general properties

The enzymatic activity versus pH is thus generally a bell-shaped curve as in Fig. 6. An autocatalytic effect may appear when the reaction products have an acid-base effect (often the case). A simple example is the glucose oxidase reaction, shown in Fig. 7. Notably, the rate versus product (H+) curve indicates an autocatalytic effect on the alkaline branch, that is, for pH>pH (see Fig. 6). Systems presenting analogous properties have been studied by R. Caplan et al.27 and we also learn more about them from D. Thomas in this volume. 

Carbonaceous substrates (graphite and glassy carbon) are generally preferred because of their mechanical, chemical, and electrochemical properties. Excellent results are also obtained by chemically modified platinum (154,156,179) and tin(IV) oxide electrodes (155). For example, glucose oxidase has been successfully immobilized by cross-linking the enzyme with BSA and GA onto an electrochemically oxidized platinum surface, with silanization using 3-amino-propyltriethoxysilane ... 

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