Glucosamine distribution

Results of chemical analysis showed that the glucosamine and total fatty acid contents of the KDO depleted, toxic lipid A and the nontoxic lipid A were essentially the same but that the nontoxic lipid A was significantly lower in the phosphorus content (Table V). The molar ratio of glucosamine phosphorus fatty acids was 2 2 4 for the toxic lipid A and 2 1 4 for the nontoxic lipid A. The relative molar distribution of normal fatty acids (lauric, myristic, and palmitic acids) and the 3-hydroxymyristic acid did not indicate a correlation between the content of these components and toxicity. The nontoxic lipid A possessed as high a tumor regression activity when combined with CWS as did the purified... 

Chitosan is produced commercially by deacetylation of chitin. It is a linear polysaccharide composed of randomly distributed /i-( l-4)-linkcd D-glucosamine (deacetylated unit) and N -acetyl-D-glucosamine (acetylated unit). The degree of deacetylation in commercial chitosans is in the range 60-100% (Figure 5.19). 

ABRF-94AAA2 was already hydrolyzed, and to provide an additional test, was spiked with glucosamine (2.0 equivalents) prior to hydrolysis. Commercially available ribonuclease B (30 i.g or 2.2 nmol, 15 p.L) and glucosamine were dissolved in water and pipetted into 6x50 mm F ex test tubes (Corning). The aliquots were dried and hydrolyzed as described [8]. The samples were analyzed by the authors and then distributed by mail, along with instructions and extensive references for analyses. 

Chitin is a polysaccharides with the exact same structure as cellulose but containing iV-acetyl-D-glucosamine or D-glucosamine and are fairly widely distributed [76] (see O Fig. 6 for the structure of a segment of chitin). It is a structural polysaccharide that forms fibers, is water impermeable, and replaces cellulose in the cell walls of many species of lower organisms. 

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