Glucitol chromatography

Certain synthetic heptitols that could not be separated by paper chromatography or by electrophoresis have been separated as their O-trimethylsilyl derivatives.404 The procedure of reductive, alkaline hydrolysis, much used in glycopeptide research, yields alditols that have been estimated as their trimethylsilyl ethers,59,405 and D-glucitol in mammalian nerve has similarly been determined.406... 

In the course of the synthesis173 of oxaprostaglandins from 1,4 3,6-dian-hydro-D-glucitol, the latter was first monotosylated at the 5-position and the ester benzylated, to afford 82. Elimination of the tosyl oxy group under special conditions yields the enolethers 83 and 84 (see Scheme 16) as a 2 1 mixture which can be separated by column chromatography. 

Fig. 18. Separation of glucitol derivatives labeled with aminobenzonitrile (ABN), obtained from methyl starch by capillary electrophoresis (CE/UV, top) and from glucitol acetates obtained from methyl cellulose by gas chromatography (GLC/FID, bottom xylitol = internal standard).

Figure 3.14 Detection of carbohydrates using PAD following isocratic separation by liquid chromatography. Peaks (1) glucitol, (2) glucose, (3) fructose (4) sucrose and (5) maltose.

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