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Gliosomes

GLUTAMATE RELEASE FROM ASTROCYTIC GLIOSOMES UNDER PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS... [Pg.295]

A. Increasing the Gliosome [C i ], by Ionomycin Induces Glutamate Release... [Pg.295]

V. Glutamate Release from Gliosomes in a Mouse Model of Amyotrophic Lateral Sclerosis... [Pg.295]

Several studies have taken advantage of the characteristics of the gliosome preparation to study functional aspects of glial cells. These studies allowed identification of specific cell distribution, function, and molecular mechanisms of a number of transmitter and modulator targets, mainly membrane transporters... [Pg.297]

The experiments shown above point out that gliosomes represent a highly purified astrocyte-derived subcellular preparation that possess the machinery to actuate exocytotic gliotransmitter release. We tested this hypothesis by monitoring directly the release of glutamate from gliosomes evoked by stimuli that augment the cytosolic ( 2r concentration [C i , by means of different mechanisms, in in vitro release experiments. [Pg.301]

Functional experiments conducted in rat cerebral cortex showed that purified gliosomes are able to take up and release glutamate when exposed to stimuli able to induce increase of intracellular Ca2+, such as ionomycin. [Pg.301]

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