Glass fibrinogen adsorption

Slack SM, Horbett TA (1988) Physicochemical and biochemical aspects of fibrinogen adsorption from plasma and binary protein solutions onto polyethylene and glass. J Colloids Interf Sci 124(2) 535-551... 

The poly (HEM A) sheets were prepared by B. Ratner using a special technique he developed. The HEMA solutions were poured between glass plates, and polymerization was chemically initiated. The chemical and physical properties of this material are very similar to those of radiation-grafted poly (HEMA) insofar as protein adsorption is concerned. Heterogeneous or homogeneous poly (HEMA) films were made by polymerization in solvents in which the poly (HEMA) is insoluble or soluble, respectively the result is a white opaque material in the first case and a transparent material in the second case. The resulting films were washed free of excess monomer and then soaked in the buffer to be used in the fibrinogen adsorption experiment for 10 days at 37 °C prior to the actual experiment. 

Fig 4. Fibrinogen adsorption c n plasma vs platelet adhesion from blood to protein coated glass for 13 minutes exposure at lOOOS-1 shear. 

Platelet adhesion did not correlate with fibrinogen adsorption to glass pretreated with a series of plasma dilutions since the experiments show the greatest number of adhering platelets did not occur where maximal fibrinogen adsorption occured. 

Maechling-Strasser, C., Dejardin, P., GaUn, J.C., Schmitt, A., 1989. Preadsorption of polymers on glass and silica to reduce fibrinogen adsorption. J. Biomed. Mater. Res. 23,1385—1393. 

ELISA and I-labelling of proteins have been seen to give results in case of fibrinogen adsorption from plasma onto glass and different copolymers. ... 

Fig. 23. Brash and ten Hove s results on the adsorption of three plasma proteins from diluted plasma as a function of total plasma concentration. Up adsorption on glass showing a maximum adsorption of fibrinogen at about 1% plasma Down adsorption on polyethylene plasma was diluted with isotonic Tris, pH 7.35. Adsorption time was 5 minutes (reprinted from Ref.1031)...

Effect of Whole Red Cells. As indicated in Figure 3, whole red cells cause a diminution in the quantity of fibrinogen adsorbed to a glass surface. The extent of this inhibition increases with increasing hematocrit, and at 40% HCT, the surface concentration after 4 h was reduced to about 50% of its value in the absence of cells. It was shown previously (11), that such an effect was not due to the depletion of fibrinogen from the aqueous phase by adsorption to the cell surfaces. Although this may occur to some extent, it is insufficient to cause any detectable alteration in the concentration of fibrino-... 

Figure 3. Adsorption of fibrinogen on glass as a function of time at various hematocrits. Conditions buffer—PBS, pH 7.35 fibrinogen concentration—1.0 mg/mL in free volume and shear rate at the surface—540 s Values are the average of at least three experiments error limits are standard deviations.

Figure 8. Adsorption of fibrinogen on glass in the presence of ghost cells. Conditions buffer—PBS, pH 7.35 and fibrinogen concentration—1.0 mglmL in free volume. Values are the average of three experiments. Key 0> ghosts at 15% HCT , ghosts at 45% HCT and —, level in absence of ghosts.

---