Gist Brocades

Microbial rennets from a number of producers, eg. Novo Nordisk, Gist Brocades, and Miles, have been available since the 1970s and have proved satisfactory for the production of different kinds of cheese. Their price is considerably lower than that of chymosin. Their properties have proven very similar to those of chymosin (89,90), and only slight modifications of the traditional cheesemaking technique are required in practice. 

Procter Gamble Gist Brocade Procter Gamble Beytout Boehr./Mann. Rubio... 

Gist-Brocades, Beverages Ingredients Group, 15 me des Comtesses, P.O.Box 239, 59472 Seclin cedex, France... 

Gist-Brocades as enzymes producer is associated to european fruit juice producers, INRA and Wageningen University Professor Voragen, in a Eureka project dealing with "Fruits liquefaction with specific enzymes". 

This research is supported by the Dutch Technology Foundation (STW), Gist Brocades and the Animal Feeds Commodity Board (PW). 

Rapidase C-80 (Gist -brocades) was used as enzyme source. The fractionation procedure of the crude preparation included chromatography on Bio-Gel PIO (100-200 mesh), DEAE-Bio-Gel A, and Bio-Gel HTP (Bio-Rad, Richmond, CA, USA). Other column materials used were cross-linked alginate (degree of cross-linking 2.34, prepared in our laboratory). Phenyl Superose HR 5/5 and Mono Q HR 5/5 (Pharmacia Biotech, Uppsala, Sweden). 

Part of this work was funded by Gist-brocades NV (Delft, The Netherlands) and the Copenhagen Pectin Factory (Copenhagen, Denmark). J.V. also acknowledges a grant of the European Community, AIR2-CT-941345. 

DSM (The Netherlands) ACF Chemie, NL Andeno, NL BMS Regensburg, De Catalytica, USA Chemie Linz, Au = Austria Deretil, E = Spain (Espana) Gist-Brocades, NL La Plaine, Switz. Wyckoff, USA... 

DSM, through the acquisition of Gist-Brocades, has a strong position in enzymes for penicillin manufacture. 

Other immobilized enzyme product successes (where annual production of immobilized enzymes has surpassed 1 ton/year) comprise Aminoacylase (Amano), hydantoinase (Smith Kline Beecham), lactase (Valio), lipase (Novo Nordisk), penicillin G acylase (Gist-brocades, Smith Kline Beecham, Rohm) and penicillin V acylase (Novo Nordisk/Gist-brocades) (Poulsen, 1984). 

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