Genomes soybean

Shoemaker, R.C. P.R. Cregan L.O. Vodkin. Soybean genomics. Soybeans Improvement, Production and Uses H.R. Boerma, J.E. Specht, Eds. American Society of Agronomy Madison, WT, 2004 pp. 235-263. 

Positive controls demonstrate adequate amplification and may be used to quantify the sensitivity of the reaction. One approach is to add known amounts of reference material [e.g., soybean and corn powder containing 0.1% (w/w) genetically altered material] to the standard PCR and to run these concurrently with the test samples. Plant genomic DNA and GMO genomic DNA may also be used as positive controls in the PCR. 

Tuteja JH, Vodkin LO (2008) Structural features of the endogenous CHS silencing and target loci in the soybean genome. Crop Sci 48 S49-S68... 

Clough SJ, Tuteja JH, Li M, Marek LF, Shoemaker RC, Vodkin LO. 2004. Features of a 103-kb gene-rich region in soybean include an inverted perfect repeat cluster of CHS genes comprising the I locus. Genome 47 819-831. 

Figure 9. (a) Soybean cellular structure. Courtesy of USDA-ARS. Special thanks to Dr. Robert Yaklich and Dr. Charles Murphy at the Soybean Genomics and Improvement Laboratory in Beltsville, MD, for creating this electron transmission micrograph especially for this chapter, (b) Crown extractor. Courtesy of Crown Iron Works, (c) Reflex extractor. Courtesy of De Smet Group, (d) LM extractor. Courtesy of De Smet Group. 

USDA-ARS, DEPARTMENT OF AGRONOMY, PURDUE UNIVERSITY, WEST LAFAYETTE, IN 47907, USA SOYBEAN GENOMICS AND IMPROVEMENT LABORATORY, USDA/ARS, BELTSVILLE, MD 20705 USA... 

Singh, R.J. T. Hymowitz. Soybean genetic resources and crop improvement. Genome 1999, 42, 605-616. 

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