Geneticin resistance selection marker

The second component of an expression vector comprises DNA sequences required for the selection, maintenance and copy-number amplification of the vector in the host cell wherein protein expression is desired. Obviously, this region is not needed for expression in E. coli. The selectable markers used in other host cells may be an enzyme conferring the ability to produce a nutrient essential for growth of the host cell, or transformation of cell morphology, or resistance to antibiotics (such as hygromycin or geneticin). Sequences for the maintenance of the vector DNA are required for the autonomous replication of the plasmid in these host cells except in cases where the vector sequences Tntegrate into chromosomes of the... 

In order to create a transfectant cell line, the desired sequence must first be cloned into an appropriate expression vector. The vector must have both a bacterial selection marker (most commonly ampidUin) and a mammaliancell selection marker (usually either geneticin (G418) or zeocin). One of the most common vectors, and the one used for this study, is the pcDNA3.1 vector, which can be purchased with either G418 or zeocin resistance. 

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