Genetically encoded small-molecule

Fluorescence or Forster resonance energy transfer (FRET) is widely accepted as being one of the most useful methods to observe biochemical events in vitro and in living cells. Generally, there are two forms of FRET sensors those based on a pair of genetically encoded fluorophores, usually employing fluorescent proteins from jellyfish or corals, or those based on small molecules that make use of small organic fluorophores. 

Translocating Sensors in Living Cells A second approach for monitoring small molecules with genetically encoded sensors is the use of translocation properties of proteins or protein domains inside cells [105]. One advantage compared to HIET sensors is the fact that only a single fusion of the translocating protein with a fluorophore, mostly a fluorescent protein, is required. Many proteins... 

Combinatorial biosynthesis, which is also known as genetic engineering of natural product biosynthetic pathways, involves a series of methods that establish novel enzyme combinations that are used for the generation of encoded libraries of bioactive small molecules [14-21]. The feasibility of this approach was first demonstrated by Hop wood et al. [22]. 

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