Genes Involved in Rebeccamycin Biosynthesis

Q Fcnretipn i)f bisindote pynqle Q Fcinttaliiin Eft carban le [ 1 iinide [iKthylatiMi 

Some of these functions were supported by heterologous expression of subsets of reb genes in Strep, albus, which resulted in production of three nonchlorinated derivatives 10-12 [22]. Therefore, the heterologous expression of indolocarbazole biosynthetic genes in an actinomycete host appeared to be a practical solution for the production of derivatives. 

Formation of bisindole pyrrole rebO staO atO Amino oxidase 

Formation of carbazole rebC staC atC Flavin-binding monooxygenase 

Sugar formation - staA atS7 Phosphosugar nucleotidyltransferase 

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