Generated DNA Probe Arrays

As a final example of DNA hybridization, k is important to mention the new possibility of DNA arrays created by a combination of photochemistry and photolithography. It is now possible to develc DNA diiqisr i ch contain tq to 15,000 different DNA sequences. This pos bili is illustrated in Hgure 21.29, which is a schematic of the synthesis of tetranucleotides by photolidtographic methods. The surface can be activated by exposure to light, allowing the desired nucleotide to be synthesized at each position on the array. These chips can be incubated with the test DNA which has been labeled with fluorescent 

F re 21.27. Release of donor quenching during polymeraM chain loactioa. Rwn Ref. 56. 

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Cronin, M.T., Fucini, R.V., Kim, S.M., Masino, R.S., Wespi, R.M. and Miyada, C.G. (1996) Cystic Fibrosis mutation detection by hybridization to hght-generated DNA probe arrays. Human Mutation, 7 244-255. 

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