Generated by Different Parts of the Chromatographic System

Dispersion Generated by Different Parts of the Chromatographic System 

The maximum allowable dispersion will include contributions from all the different dispersion sources. Furthermore, the analyst may frequently be required to place a large volume of sample on the column to accommodate the specific nature of the sample. The peak spreading resulting from the use of the maximum possible sample volume is likely to reach the permissible dispersion limit. It follows that the dispersion that takes place in the connecting tubes, sensor volume and other parts of the detector must be reduced to the absolute minimum and, if possible, kept to less than 10% of that permissible (i.c.,1 % of the column variance) to allow large sample volumes to be used when necessary. 

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