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General Lead Identification Strategies

The second, so-called mechanism-based approach tries to optimize target activity. This procedure relies on a suitable biochemical assay to study target protein function in the presence of screening compounds. The challenge for [Pg.77]

Summing up the various MS tools discussed leads to a general protocol for metabolite identification. It can be argued that such a protocol should imply the use of various types of mass spectrometers. In a nice paper, Clarke et al. [48] outlined such a multi-instrument strategy, which after slight modification comprises of ... [Pg.273]

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