General Comments on Optimizing the 2DLC Experiment Speed-Resolution Trade-off

6 GENERAL COMMENTS ON OPTIMIZING THE 2DLC EXPERIMENT SPEED-RESOLUTION TRADE-OFF 

As with any separation technique, the desired goal is to maximize peak resolution at the fastest speed. Higher resolution in 2DLC is easier to achieve than when using onedimensional chromatography because selectivity differences between the two different columns can give a resolution enhancement. This is easily seen through the simplified resolution equation, discussed in Chapter 2, 

Equation 6.1 demonstrates that if resolution is low in one of the dimensions and high in the other dimension, the result is at least as large as the higher resolution. However, limited sampling of the first dimension can make the standard deviation of the zone in 

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