Gene manipulation reaction

Yamamoto Y, Uehara A, Watanabe A, Aburatani H, Komiyama M. Chemical-reaction-based site-selective DNA cutter for PCR-free gene manipulation. ChemBioChem. 2006 7 673-677. 

In summary, the cyclohexanediones and aryloxyphenoxypropionates are herbicidal because they inhibit the carboxyltransferase partial reaction of acetyl-CoA carboxylase in sensitive plants. Their selectivity appears to be based on structural differences in the target enzyme, and this raises fundamental questions about protein sequence and conformation as well as offering some attractive possibilities for gene manipulation. ... 

The CFCF described by Spirin (30) is efficient for protein production using the efficient wheat germ cell-free system, as shown in the previous section. However, this system may not be suitable for massive screening of cDNA libraries for structural genomics and production of gene products. Because the CFCF apparatus is equipped with a semipermeable membrane in the reaction chamber, the system is quite complicated to manipulate. The delicate nature of the membrane can pose problems for automating the translation process. Also,... 

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