Gene delivery systems polymer-based

Nonviral vector systems are usually either composed of a plasmid based expression cassette alone ( naked DNA), or are prepared with a synthetic amphipathic DNA-complexing agent (84, 88). Gene delivery systems based on nonviral vectors mainly comprise cationic liposomes, DNA-polymer-protein complexes, and mechanic administration of naked DNA. An idealized/optimized multifunctional nonviral gene delivery system is depicted in Figure 13.4. 

Cationic Polymer-Based Gene Delivery Systems... 

Brownlie, A., Uchegbu, I.F., Schatzlein, A.G. (2004). PEI-based vesicle-polymer hybrid gene delivery system with improved biocompatibility. Int. J. Pharm., 274 (1-2), 41-52. 

Chemg, J.Y., van de Wetering, P., Talsma, H., Crommelin, D.J. and Hennink, W.E. (1999b) Stabilization of polymer-based gene delivery systems. Int. J. Pharm., 183, 25-28. 

Outline the various types of polymer-based gene delivery systems. 

Though less studied than synthetic polymer and lipid-based gene delivery systems, there have been significant advances in the field of natural polymers for gene delivery. Biodegradable polymers are less likely to accumulate within tissues than their synthetic counterparts. Synthetic polymers are not easily removed by normal clearance systems and can accumulate in tissues. 

Smedt S C, Demeester J, Hennink W E (2000). Cationic polymer based gene delivery systems. Pharm. Res. 17 113-126. 

Despite the advances in polymer-based gene delivery systems in order to achieve an optimal balance between the conflicting requirements for DNA protection during the transport and the efficient release into the cells, more complex delivery vehicles have been developed. 

Cationic Polymer-Based Non-viral Gene Delivery Systems and their Application in Gene-Engineered Stem Cells... 

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