Gene delivery methods

Kaneda, Y., Yamamoto, S., Hiraoka K. (2003). The hemagglutinating virus of Japan— liposome method for gene delivery. Methods Enzymol., 373, 482-493. 

The necessity of more efficient gene delivery methods prompted the search for novel, less charged or non-cationic gene delivery systems. These non-electrostatic complexes can be advantageous for in vitro and in vivo applications, since unlike cationic lipid/DNA complexes, the novel molecules could not lead to a compacted state of DNA, and could therefore potentially lead to different kinetics of DNA release from complexes. Several compounds are able to bind to double stranded DNA along the grooves by the formation... 

Eliltunen, M.O., Turunen, M.P., Turunen, A.M., Rissanen, T.T., Laitinen, M., Kosma, V.M. etal. (2000) Biodistribution of adenoviral vector to non-target tissues after local in vivo gene transfer to arterial wall using intravascular and periadventitial gene delivery methods. FASEB J., 14, 2230-2236. 

Green JJ, Zugates GT, Langer R et al (2009) Poly(beta-amino esters) procedures for synthesis and gene delivery. Methods Mol Biol 480 53-63... 

Although promising, NP-based gene delivery methods have not yet been applied to mammalian embryos. However, a few attempts have been made to use NP-based gene delivery methods in species in which conventional methods of gene... 

Liposomes Liposome-based gene transfer is anonviral gene delivery method that uses the combination of phospholipids, cholesterol, and some polymers for the formation of spherical vesicles. DNA is dehvered into cells by liposomes via membrane fusion and endocytosis. This method has several advantages such as enhanced delivery, nontoxicity, the protection of nucleic acids from nuclease activity, and the abihty to target DNA into a variety of ceU types, including protoplasts, via plasmodesmata. 

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