Gene delivery fusogenic liposome

Yuba E, Kojima C, Sakaguchi N, Harada A, Koiwai K, Kono K (2008) Gene delivery to dendritic cells mediated by complexes of lipoplexes and pH-sensitive fusogenic polymer-modified liposomes. J Control Release 130 77-83... 

Modification of depolymerization kinetics and release Endosomal escape Nonviral gene delivery Boron neutron capture therapy Fusogenic liposomes, increase transfection efficiency... 

Several reports in the literature state that DOPE, while successfully used for in vitro gene delivery, is a poor helper lipid for in vivo applications [28-32], Instead, for reasons that are not understood, lipid mixtures for successful transfection in vivo seem to require cholesterol [33]. In fact, an equimolar mixture of cholesterol and DOTAP is widely used for in vivo experiments and clinical trials. Cholesterol has also been included in liposomes along with cationic DOTAP and fusogenic DOPE to form a potent mixture used to study the treatment of ovarian cancer by delivery of the p53 tumor suppressor gene [34, 35]. 

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