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GC-rich primers

Fignre Z The Polymerase Chain Reaction temperature cycle. The exact temperatures depend upon the type of primers, AT rich primers denature more easily than GC rich primers... [Pg.1135]

Fig. 2. Sets of overiap sequences for fusion PCR. Each set of primers, such as 15C and 15G, should be used together to amplify the two fragments to be fused, one at the 5 end and another at the 3 end. Type 1 sequence is used for fusions that allow mutations in 15C/15G region (see Note 2). Type 2 sequence is useful for in-frame fusion owing to its low mutation frequency. Type 3 sequence Is used for GC-rich templates that sometimes prevent amplification with GC-rich primers. Fig. 2. Sets of overiap sequences for fusion PCR. Each set of primers, such as 15C and 15G, should be used together to amplify the two fragments to be fused, one at the 5 end and another at the 3 end. Type 1 sequence is used for fusions that allow mutations in 15C/15G region (see Note 2). Type 2 sequence is useful for in-frame fusion owing to its low mutation frequency. Type 3 sequence Is used for GC-rich templates that sometimes prevent amplification with GC-rich primers.
See other pages where GC-rich primers is mentioned: [Pg.94]    [Pg.52]   
See also in sourсe #XX -- [ Pg.52 ]



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