Ganglioside binding

Fig. 8.7 CTB-GM1-ganglioside binding ELISA assay. Plates, coated first with GMrganglioside and bovine serum albumin (BSA), respectively, were irrigated with total soluble plant protein from chloroplast transgenic lines (3 and 7) and 300 ng of purified bacterial CTB. The absorbance of the GM1-ganglioside-CTB-antibody complex in each case was measured at 405 nm. Total soluble protein from untransformed plants was used as the negative control.

Two of the most widely spread and well-studied enterotoxigenic forms of bacterial diarrhea are ETEC and Vibrio cholerae. The toxins they produce, labile toxin (LT) and cholera toxin (CT) respectively, are very similar in primary sequence, structure, and mechanism of action [72]. They are homologous multi-subunit proteins in which the non-toxic B subunit mediates GMj ganglioside binding, and thus are candidates for vaccines that can neutralize toxin activity. 

Studies currently in progress are critically analyzing the characteristics of ganglioside binding to PBMC and relating them to functional activity. 

Table 4 Comparison of ganglioside binding of SabA-expressing H. pylori strains and H. pylori neutrophil-activating protein HP-NAP...

Nawar HF, Arce S, Russell MW ct aL Mutants of Type II heat-labile enterotoxin LT-IIa with altered ganglioside-binding activities and diminished toxicity arc potent mucosal adjuvants. Infect Immun 2007 75(2) 621-633. 

Yahi N, Fantini J. Deciphering the glycolipid code of Alzheimer s and Parkinson s amyloid proteins allowed the creation of a universal ganglioside-binding Peptide. PloS One. 2014 9(8) el04751. 

CHOLESTEROL- AND GANGLIOSIDE-BINDING DOMAINS IN SEROTONIN RECEPTORS... 

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