Galactosyl transferases location

When the A-tosylaziridine 11 was opened with fluoride (KHFj, DMF, 150°C) initially the 2-fluoride 12 was produced, but over time the thermodynamic product 13 formed, presumably by reversal back to 11. This reaction was also utilized in the synthesis of fluorinated kanamycin derivatives." These and other syntheses of 4 -deoxy-4 -fluorokanamycins A and B are covered in Chapter 19. The synthesis of a fluorinated analogue of the acceptor of bovine (l- 4)-P-D-galactosyl transferase is mentioned in Chapter 3, and the conformational analysis of some deoxyfluoro sugars by H-n.m.r. spectroscopy is detailed in Chapter 21. The use of e.i. and c.i. mass spectrometry to determine the location of a fluorine atom in deoxyfluoro glucosides is outlined in Chapter 22. 

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