3-Galactosidase synthesis

In the presence of 100 pg/m adenine, /3-galactosidase synthesis was repressed by more than 90%. After removal of the adenine there was a lag of about 6-8 minutes before j3-galactosidase activity could be detected, after which its activity increased linearly for at least 30 minutes. 

One of the current ideas is that RNA has a regulatory, rather than a direct function in protein synthesis. For example, the repressor of /3-galactosidase synthesis in inducible strains of E. coli might be RNA,... 

P-Glycosidase and [5-galactosidase Synthesis of alkyl-b-glucosides in acetate buffer/l-hexanol or 1-octanol [67]... 

Galactosidase Synthesis Is Augmented by a Small-Molecule Inducer... 

Zubay, G., M. Lederman, and J. DeVries, DNA-directed peptide synthesis III. Repression of jS-galactosidase synthesis and inhibition of repressor by inducer in a cell-free system. Proc. Natl. Acad. Sci. USA 58 1669-1675, 1967. Showing that repressor works in a cell-free system. 

Mosbach K, Guilford H, Ohlsson R et al. (1972) General Ugands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5 -monophosphate and nicotinamide-adenine dinucleotide. Biochem J 127(4) 625-631 Moses V, Prevost C (1966) Catabolite repression of P-galactosidase synthesis in Escherichia coli. Biochem J 100(2) 336-353... 

A number of workers have shown that novobiocin inhibits protein synthesis in bacteria. For example, it inhibits /S-galactosidase synthesis in both Staph, aureus and E. coli [48, 64]. Although M-protein synthesis in a Group A streptococcus is not inhibited by novobiocin [70], the inhibition of protein synthesis in Strept. faecium has been attributed to the inhibition of tRNA synthesis [23]. Novobiocin inhibits protein synthesis in Staph, aureus [26], but as the inhibition of other macro-molecules also occurs to a similar extent, this effect may not be the primary action of the antibiotic. The antibiotic also inhibits protein synthesis in E. coli [33, 71] but this inhibition appears much later than the observed inhibition of deoxyribonucleic acid (DNA) synthesis. 

Perlman and Pastan [20] also showed that cyclic AMP affected the production of galactosidase. In bacteria made permeable by EDTA, 10 Af cyclic AMP increased the production of galactosidase by amounts ranging from 30 to 100%, and this stimulation was not accompanied by an increase in either total RNA or protein synthesis. These workers found, as did Ullman and Monod, that cyclic AMP could overcome transient repression of galactosidase synthesis in normal strains, constitutive strains, and cryptic mutant strains. Furthermore, Perlman and... 

Perlman, R. L., Pastan, I Regulation of j5-galactosidase synthesis in Escherichia coli by cyclic adenosine-3, 5 -monophosphate. J. biol. Chem. 243, 5420-5427 (1968b). 

Zubay, G., Chambers, D. A. A DNA-directed cell-free system for j5-galactosidase synthesis characterization of the novo S5mthesized enzyme and some aspects of the regulation of s)nthesis. Cold Spr. Harb. Symp. quant. Biol. 34, 753-761 (1969). 

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