Galactose liquid chromatography

Some workers have performed hydrolyses in an autoclave, but it has been shown that 0.5 M sulfuric acid at 120° degrades 33% of L-arabinose and 22% of D-galactose in two hours.75 Such methods are, therefore, only suitable for qualitative analyses, unless accurate corrections are made. Similarly, 90% formic acid has been found76 to decompose 48% of D-xylose and 36% of D-galactose in 20 hours at 100°. Gas-liquid chromatography has been used to examine the products formed by the acid degradation of sugars.77... 

There have been several reports of the separation of a- and / -pyranose forms during liquid chromatography of sugars.46-49 Initially, this was regarded as a nuisance, but systematic attempts were later made to improve this separation. At 0°, with 1 4 water-acetonitrile as the eluant, many aldoses were separated into two components.48 Galactose and al-trose showed small peaks probably representing the furanose forms ... 

R. Takano, K. Kamei-Hayashi, S. Hara, and S. Hirase, Assignment of the absolute configuration of partially methylated galactoses by combined gas-liquid chromatography—mass spectrometry, Biosci. Biotech. Biochem., 57 (1993) 1195—1197. 

CIO. Copenhaver, J. H., Quantitative analysis of plasma galactose and glucose by gas-liquid chromatography. Anal. Biochem. 17, 76-83 (1966). 

Gas-Liquid Chromatography. — All reports are concerned with the use of crq>illaiy columns. G.c.-(e.i.)m.s. analysis of partially methylated alditol acetate derivatives has been used in the study of water soluble pectins, and the partially ethylated alditol and aldononitiile acetate derivatives of galactose and mono-O-methyl-galactoses have been similarly examined. ... 

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