Galactosamine phosphorylation

Cardini, C. E. and Leloir, L. F. 1952. Enzymic phosphorylation of galactosamine and galactose. Arch. Biochem. Biopkys. 45, 55-64. 

Figure 3.234 Gradient elution of phosphoryl-ated mono- and disaccharides. Separator column CarboPac PA1 eluent (A) 0.1 mol/L NaOH, (B) 0.1 mol/L NaOH+ 1 mol/L NaOAc gradient linear, 10% B to 20% B in 20 min and then to 50% B in 10 min flow rate 1 mL/min detection pulsed amperometry on a gold working electrode injection volume 50 pL peaks 22.6 mg/L a-D-galactosamine-1-P (1),...

Figure 3.269 Separation of hydrolysate amino acids and O-phosphorylated amino acids on AminoPac PA10. Eluent NaOH/NaOAc gradient graphically depicted flow rate 0.25 mU min detection see Figure 3.268 chromatogram (a) 200pmol each of arginine (1), hydroxylysine (2), lysine (3), galactosamine (4), glucosamine (5), alanine (6), threonine (7),...

A type of reaction which is applicable in many cases is the phosphorylation with ATP using different kinases. In this manner many esters can be obtained from the free sugars such as glucose 6-phosphate, mannose 6-phosphate, fructose i-phosphate, fructose 6-phosphate, fructose i,6-diphosphate, galactose i-phosphate, galactosamine i-phosphate, gluconic acid 6-phos-phate, ribose 5-phosphate and ribulose 5-phosphate. A special case of enzymic phosphorylation in which a pyrophosphate residue is transferred from ATP is the following reaction ... 

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