Functionalizing G3-MNP with Glutaraldehyde

G3-MNP (150 mg) were suspended in glutaraldehyde (25 %, 20 mL) and sonicated for one hour. After 12 h standing the nanoparticles were separated from glutaraldehyde and washed with TRIS buffer pH 8 (5 x 15 mL). G3-MNP-glutaraldehyde were used in the next step without further purification for the GpdQ immobilization and for the ligand immobilization they were washed with methanol (1 mL). 

To G3-MNP-glutaraldehyde (75 mg) in methanol (1 mL) was added NH2HL2 (50 mg) and dried potassium carbonate (75 mg) and the mixture was left standing at room temperature for live days. After this time the nanoparticles were washed by magnetic separation until the solution was clear. The nanoparticles were then resuspended in methanol (1.5 mL) and zinc acetate dihydrate (50.2 mg) was added. After stirring for 30 min the MNP were washed with methanol (5 X 1.5 mL) and dried in vacuo. 

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