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Function studies, HPLC method

Thus, the analytical HPLC method for glycolipids is proving useful for a variety of studies related to glycosphingolipid function and metabolism. [Pg.5]

This chapter discussed the-use of the HPLC method to assay multiple enzyme activities and individual enzymes with more than one function. Examples were given to illustrate that the method can be used to assay two activities using the same substrate to form different products, two activities using the same substrates to form the same product, and one activity using two substrates to form two products. Also, the HPLC method can be used to study an in vitro multienzyme complex reconstructed by the addition of pure enzymes and finally to study a naturally occurring multienzyme complex. [Pg.435]

The application of the improved MS techniques presented above with highly resolving separation methods, such as 2-D electrophoresis, capillary HPLC, and CE, resulted in the creation of a new science, proteomics63 While genomics, described by DNA databases, represents the ground stage of the cell, the study of the differential status of the cell, due to various stimuli or disease states, reflects the functional expression of protein products or proteomics. Proteomics studies are aimed at identifying the proteome, the network of proteins that define the... [Pg.233]

Ahmad S, Ahsan A, Iqbal O, et al. Pharmacokinetics and pharmacodynamics of argatroban as studied by HPLC and functional methods implications in the monitoring and dosage-optimizations in cardiovascular patients. Clin Appl Thromb Hemost 1998 4 243-249. [Pg.106]

The elucidation and confirmation of structure should include physical and chemical information derived from applicable analyses, such as (a) elemental analysis (b) functional group analysis using spectroscopic methods (i.e., mass spectrometry, nuclear magnetic resonance) (c) molecular weight determinations (d) degradation studies (e) complex formation determinations (f) chromatographic studies methods using HPLC, GC, TLC, GLC (h) infrared spectroscopy (j) ultraviolet spectroscopy (k) stereochemistry and (1) others, such as optical rotatory dispersion (ORD) or X-ray diffraction. [Pg.195]

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HPLC methods

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