Fruits, anthocyanins water content

Serial dilutions are recommended to ensure accurate measurements of highly concentrated, high density, or dried samples. Perform a weight-by-volume dilution with distilled water to obtain a single-strength solution (e.g., usually around 10° Brix for fruit juices unit hi,4), followed by a second dilution using 0.025 M potassium chloride buffer, pH 1.0. Both dilution factors must be considered when calculating monomeric anthocyanin content. 

Silver nanoparticles using black elderberry fruit extracts were synthesized and their antiinflammatory effects were evaluate [51]. The anthocyanins from elderberries were extracted with a solvent mixture (acetone-water 4 1) at room temeperature. In order to obtain a silver nanomaterial a silver salt solution was mixed and boiled with 16.6 mL fruit extract (total anthocyanin content 24 x 10-3mM). The synthesized nanoparticles presented a promising anti-inflammatory effeet, investigated both in vitro and in vivo. In vitro, the anti-inflammatory effect was demonstrated by the decrease of cytokines production and by maintaining their low level after UVB irradiation. In vivo, the pre-administration of silver nanoparticles decreased the level of cytokines in the paw tissues and also presented long-term protective effect. The local treatment of psoriasis vulgaris skin lesions confirmed the good anti-inflammatory effect of silver nanoparticles, which proved to be even better than that of hydrocortisone. 

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