Fructose loading test

Oral fructose loading test is not advocated in the case of suspicion of hereditary fructose intolerance since oral administration of fructose may cause severe gastrointestinal symptoms. 

Indication while dangerous and obsolete in the diagnosis of HFI, the oral fructose loading test serves as a reference test in the diagnosis of SGLTl deficiency. 

Initiation/Termination Control of Enzyme Reaction. The enzymatic hydrolysis of sucrose was studied at 25 C through a monitoring of the formation rate of reducing sugar products (an equimolar mixture of glucose and fructose) in an aqueous suspension of the enzyme-loaded capsules. The suspension (50 mL) initially contained 100 mM of the substrate and 13.5% (v/v) of the capsules with a total of 200 mg of the encapsulated enzyme. On/off control was tested by investigating the batch reaction kinetics at pH 5.5 (at which the reaction occurs) and at pH 4.5 (at which the reaction stops). The adjustment of pH was made by quick additions of a small amount of 2M HCl or NaOH. 

---