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Freeze clamp method

In general, it appears that fixation by fast freezing, (for example, by clamping between aluminum blocks chilled in liquid nitrogen), followed by pulverisation in impaction mortars chilled to the temperature of liquid nitrogen, and finally rapid homogenisation of the frozen, powdered tissue in 0.1 N HCl, containing radioactively labelled cyclic nucleotides to determine recovery, is the most reliable method of fixation. [Pg.313]


See other pages where Freeze clamp method is mentioned: [Pg.307]    [Pg.307]    [Pg.307]    [Pg.307]    [Pg.307]    [Pg.307]    [Pg.248]    [Pg.339]    [Pg.82]    [Pg.617]    [Pg.82]    [Pg.170]    [Pg.1061]    [Pg.1061]    [Pg.257]    [Pg.170]    [Pg.59]    [Pg.59]    [Pg.531]    [Pg.272]    [Pg.162]    [Pg.80]    [Pg.224]    [Pg.313]    [Pg.150]    [Pg.16]    [Pg.52]   
See also in sourсe #XX -- [ Pg.307 ]

See also in sourсe #XX -- [ Pg.307 ]

See also in sourсe #XX -- [ Pg.307 ]




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Clamping

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Freeze-clamping

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