Free-solution conjugate electrophoresis

DNA Sequencing and Genotyping by Free-Solution Conjugate Electrophoresis... 

Free-solution electrophoresis of DNA that has been conjugated to a friction-perturbing entity, which we call a drag-tag, was not feasible until after the development of CE in the 1990s. Free-solution electrophoresis generates a significant amount of heat, which must be dissipated. The narrow channels used in CE have diameters in the order of 25-100 p,m, which efficiently remove this Joule heat. Free-solution conjugate electrophoresis (FSCE) was first examined quantitatively in 1994 by Mayer... 

Vreeland, W. N. et al. Molar mass profiling of synthetic polymers by free-solution capillary electrophoresis of DNA-polymer conjugates. AnaZyficaZ Chemistry 13, 1795-1803 (2001). 

Vreeland, W. N., Slater, G W., and Barron, A. E. Profiling sohd-phase synthesis products by free-solution conjugate capillary electrophoresis. Bioconjugate Chemistry 13, 663-670 (2002). 

Meagher, R. J. et al. Multiplexed p53 mutation detection by free-solution conjugate microchannel electrophoresis with polyamide drag-tags. Analytical Chemistry 79, 1848-1854 (2007). 

McCormick, L. C. and Slater, G W. The molecular end effect and its critical impact on the behavior of charged-uncharged polymer conjugates during free-solution electrophoresis. Electrophoresis 26, 1659-1667 (2005). 

McCormick, L.C. and G.W. Slater, Molecular deformation and free-solution electrophoresis of DNA-uncharged polymer conjugates at high field strengths Theoretical predictions. Part 1 Hydrodynamic segregation. Electrophoresis, 2007 28(4) 674-682. 

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