Fragmentation nucleic acids

As discussed in Chapter 11, electrophoresis refers to a group of techniques used to separate and study molecules with electrical charges. Based upon these charges, both sign and magnitude, biomolecules such as proteins, peptides, amino acids, nucleic acids, and fragmented nucleic acids migrate in an electrical... 

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Yakovlev AG, Wang G, Stoica BA et al. Role of DNAS1L3 in Ca2+- and Mg2+-dependenl cleavage of DNA into oligonucleosomal and high molecular mass fragments. Nucleic Acids Res 1999 27(9) 1999-2005. 

Nucleic acid fragments Nucleic acid-binding proteins... 

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Fig. 1. Southern blot analysis of DNA showing (a) step 1, an agarose gel containing separated restriction fragments of DNA, denoted by (—), which is immersed in NaOH to denature the double-stranded stmcture of DNA, and then transferred by capillary flow to a nitrocellulose filter. In step 2, the bound DNA is allowed to hybridize to a labeled nucleic acid probe, and the unbound probe is washed off In step 3, the filter is placed into contact with x-ray film resulting in (b) bands of exposure on the film which are detected after development and correspond to regions where the restriction fragment is...

Nucleic acid (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)) probes utilize labeled, ie, radioactive, enzymatic, or fluorescent, fragments of DNA or RNA (the probe) to detect complimentary DNA or RNA sequences in a sample. Because the probe is tailored for one specific nucleic acid, these assays are highly specific and very sensitive (45). 

Oligosaccharides Nucleic acids DNA fragments TSK-GEL G-Oligo-PW, TSK-GEL G2000PW TSK-GEL G2500PWxl Small particles, high resolving power... 

Most reactions of nucleic acid hydrolysis break bonds in the polynucleotide backbone. Such reactions are important because they can be used to manipulate these polymeric molecules. For example, hydrolysis of polynucleotides generates smaller fragments whose nucleotide sequence can be more easily determined. 

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