Fragment-oriented design

We envisage that the insights discussed in this chapter provide chemists with better design principles, and therefore contribute to a more rational fragment-oriented design of catalysts. 

Diefenbach A, De Jong GT, Bickelhaupt FM (2005) Fragment-oriented design of catalysts based on the activation strain model. Mol Phys 103 995... 

In this chapter, we address two complimentary approaches to componnd collection development namely, Diversity Oriented Synthesis (DOS) and Biology Oriented Synthesis (BIOS). For additional, very well-validated approaches snch as fragment-based design and the application of in silico methods to develop compound libraries the reader is referred to different chapters in this book and to authoritative reviews. " ... 

One of the most important features in the immunosensor design is the proper choice of the immobilization method for keeping the affinity of the antibodies. As was previously demonstrated for Protein A, when the antibodies are immobilized through their Fc fragment to Protein A (or G), their Fab binding sites are mostly oriented away from the solid phase. As Protein A is able to link the Fc region of different antibodies, there is no need to modify the antibody with biotin. As an antecedent, we have previously demonstrated the utility of Protein A biocomposite (ProtA-GEB) for the universal attachment of antibodies with different specificities [54]. 

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