FRACTIONATION Subject

Chapter 13 - It was shown, that limiting conversion (in the given case - imidization) degree is defined by purely structural parameter - macromolecular coil fraction, subjected evolution (transformation) in chemical reaction course. This fraction can be correctly estimated within the framework of fractal analysis. For this purpose were offered two methods of macromolecular coil fractal dimension calculation, which gave coordinated results. 

The biochemical fractions of the serum alkaline phosphatase in cirrhosis are listed in Table 13 according to blood types A and 0. It may be advantageous to compare the various moieties in these patients with the data on normal subjects of corresponding blood type (Table 10). In some cases (4, 5, 14) the elevation is proportionate in the LPSAP and non-LPSAP moieties, whereas in others (1, 2, 3, 9, 10, 12, 13, 15) there is a disproportionate elevation in the LPSAP fraction. Subject 7 exhibits the largest contribution of LPSAP (60%). There appears to be no similarity of the heat sensitivities of LPSAP and non-LPSAP in subjects 4, 5, 7, 14, and 15 with total LPSAP hyperphosphatasemia. In fact, the relatively high heat inactivation of sera 5, 6, 7, and 12 would register negatively for liver in a heat-inactivation test of total alkaline phosphatase (P19). 

Scheil and Harris [137] developed a system for the accurate determination of C H ratios in chromatographically separated peaks. A proportioning tap feeds fractions subjected to the analysis into a reactor filled with copper oxide, where oxidation takes place at 700°C. The oxidation products are separated on Porapak N. The results of the analysis were evaluated by a computer. The method was applied to compounds containing up to 12 atoms of carbon. If reference compounds of a similar structure are available, the method can reduce the mean error of determining C H ratios to 0.2%, which corresponds to the absolute error of carbon and hydrogen determination (0.02—... 

This is illustrated by Fig. 25, where media with various final values of pH were used (from the left) 3.4, 3.6, 3.7, 3.8, 3.9, 4.0 and 4.2 it is apparent that the range 3.7-3.9 is satisfactory. Just as in the examinations of pepsinogen, we also added cathepsin to electrophoretic protein fractions, and here the same result was obtained, showing that albumin is the principal fraction subject to polarographically detectable cleavage. We believe that the explanation is the same as that valid for pepsinogen, as described in Section 11. 

Fig. lA-D. Immunofractionation of chromatin digested by micrococcal nuclease for various extents of time on an anti-poly(ADP-ribose) Sepharose column. HeLa nuclei from 10 cells were digested with micrococcal nuclease [50 units/10 nuclei at 37°C for 1 min (A), 5 min (B), 10 min (C), and 20 min (D)]. Soluble chromatin was subsequently prepared and immunofractionated on an anti-poly(ADP-ribose) Sepharose column. The columns were loaded and washed with phosphate buffered saline. Transmittance at 254 nm was continuously monitored. When no further UV-absorbing material was eluted, 6 M guanidinium hydrochloride was added to elute-bound material. The insets in each panel represent 1/50 of the DNA extracted from the pooled unbound U) and bound (B) peak fractions subjected to electrophoresis on 1.5% agarose. The gel was stained with ethidium bromide. (Taken from [10])... 

FIGURE 4.12 The dependences of elastically deformed regions fraction % (1, 2) and crystallites fraction, subjected to partial melting, (3, 4) on testing temperature T in impact (1,3) and quasistatic (2,4) tests for HDPE [51],... 

The former technique consists of the manufacture of extruded or injected products, whose main component is either PE or PVC, whereas other plastics act as fillers. The latter consists of separation of the mix of collected plastics into homogeneous fractions, subjected to a recycling process which brings their characteristics and purity as near as possible to those of original polymers. The two techniques are not contrasting, in fact they can be regarded as complementary to each other. 

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