Fractionation Methods Before the Use of Infrared Spectroscopy

Many fractionation techniques have been refined in recent years. This chapter has the purpose of naming types of compounds that can be separated by some of the methods commonly used for the isolation of biochemical substances or for the examination of their purity. Discussions of theory, equipment, and applications will be found in many of the references given in this chapter. 

Rosenkrantz (1957) has written one of the earlier articles on the utilization of fractionation procedures with infrared analysis and has listed several types of fractionation techniques chromatography, countercurrent distribution, preferential solvent extraction, sublimation, fractional crystallization, molecular distillation, dialysis, centrifugation, electrophoresis, diffusion, and freeze-drying. He has also given references to work in which these methods have been used to fractionate a large variety of biological compounds. Elvidge and Sammes (1966) have discussed many of the techniques mentioned above. 

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